PREPRINT: Factors affecting CRISPR-Cas defense against antibiotic resistance plasmids harbored by Enterococcus faecalis laboratory model strains and clinical isolates

Buttaro BA, Palmer KL. Factors affecting CRISPR-Cas defense against antibiotic resistance plasmids harbored by Enterococcus faecalis laboratory model strains and clinical isolates. bioRxiv [Preprint]. 2025 Mar 10:2025.03.10.642232. doi: 10.1101/2025.03.10.642232. PMID: 40161755; PMCID: PMC11952401.

Phosphorylation of (Ser 291) in the linker insert of Syk negatively regulates ITAM signaling in platelets
Dangelmaier C, Vari HR, Vajipayajula DN, Elzoheiry M, Wright M, Iyer A, Tsygankov AY, Kunapuli SP. Phosphorylation of (Ser 291) in the linker insert of Syk negatively regulates ITAM signaling in platelets. Platelets. 2024 Dec;35(1):2369766. doi: 10.1080/09537104.2024.2369766. Epub 2024 Jun 21. PMID: 38904212; PMCID: PMC11322839. 

Phosphorylation of (Ser 291) in the linker insert of Syk negatively regulates ITAM signaling in platelets
Dangelmaier C, Vari HR, Vajipayajula DN, Elzoheiry M, Wright M, Iyer A, Tsygankov AY, Kunapuli SP. Phosphorylation of (Ser 291) in the linker insert of Syk negatively regulates ITAM signaling in platelets. Platelets. 2024 Dec;35(1):2369766. doi: 10.1080/09537104.2024.2369766. Epub 2024 Jun 21. PMID: 38904212; PMCID: PMC11322839.

Distinct Role of GRK3 in Platelet Activation by Desensitization of G Protein-Coupled Receptors
Chaudhary PK, Kim S, Kunapuli SP, Kim S. Distinct Role of GRK3 in Platelet Activation by Desensitization of G Protein-Coupled Receptors. Thromb Haemost. 2024 Nov 11. doi: 10.1055/a-2442-9031. Epub ahead of print. PMID: 39419098.

Recent advances in vascular thiol isomerases and redox systems in platelet function and thrombosis
David W Essex, Lu Wang. Recent advances in vascular thiol isomerases and redox systems in platelet function and thrombosis J Thromb Haemost. 2024 Jul;22(7):1806-1818. PMID: 38518897

Thioredoxin-related transmembrane protein 1 negatively regulates coagulation and phosphatidylserine exposure
Zhou J, Rico MC, Rauova L, Poncz M, Essex DW. Thioredoxin-related transmembrane protein 1 negatively regulates coagulation and phosphatidylserine exposure. Res Pract Thromb Haemost. 2024 Jun 11;8(4):102472. doi: 10.1016/j.rpth.2024.102472. PMID: 39036672; PMCID: PMC11260325. 

Role of Tula-Family Proteins in Cell Signaling and Activation: Advances and Challenges

Tsygankov AY. Role of Tula-Family Proteins in Cell Signaling and Activation: Advances and Challenges. Int J Mol Sci. 2024 Apr 18;25(8):4434. doi: 10.3390/ijms25084434. PMID: 38674019; PMCID: PMC11050124.

Biochemical characterization of spleen tyrosine kinase (SYK) isoforms in platelets
Singh P, Dangelmaier CA, Reddy Vari H, Tsygankov AY, Kunapuli SP. Biochemical characterization of spleen tyrosine kinase (SYK) isoforms in platelets. Platelets. 2023 Dec;34(1):2249549. doi: 10.1080/09537104.2023.2249549. PMID: 37661351; PMCID: PMC10502920.

Novel Strategy to Combat the Procoagulant Phenotype in Heparin-Induced Thrombocytopenia Using 12-LOX Inhibition
Renna SA, Zhao X, Kunapuli SP, Ma P, Holinstat M, Boxer MB, Maloney DJ, Michael JV, McKenzie SE. Novel Strategy to Combat the Procoagulant Phenotype in Heparin-Induced Thrombocytopenia Using 12-LOX Inhibition. Arterioscler Thromb Vasc Biol. 2023 Oct;43(10):1808-1817. doi: 10.1161/ATVBAHA.123.319434. Epub 2023 Jun 22. PMID: 37345522.

Phosphorylation of spleen tyrosine kinase at Y346 negatively regulates ITAM-mediated signaling and function in platelets
Dangelmaier CA, Patchin M, Vajipayajula DN, Vari HR, Singh PK, Wright MN, Kostyak JC, Tsygankov AY, Kunapuli SP. Phosphorylation of spleen tyrosine kinase at Y346 negatively regulates ITAM-mediated signaling and function in platelets. J Biol Chem. 2023 Jul;299(7):104865. doi: 10.1016/j.jbc.2023.104865. Epub 2023 Jun 1. PMID: 37268160; PMCID: PMC10320515.

TULA Proteins in Men, Mice, Hens, and Lice: Welcome to the Family
Tsygankov AY. TULA Proteins in Men, Mice, Hens, and Lice: Welcome to the Family. Int J Mol Sci. 2023 May 23;24(11):9126. doi: 10.3390/ijms24119126. PMID: 37298079; PMCID: PMC10253181.

Activating P2Y1 receptors improves function in arteries with repressed autophagy
Cho JM, Park SK, Kwon OS, Taylor La Salle D, Cerbie J, Fermoyle CC, Morgan D, Nelson A, Bledsoe A, Bharath LP, Tandar M, Kunapuli SP, Richardson RS, Anandh Babu PV, Mookherjee S, Kishore BK, Wang F, Yang T, Boudina S, Trinity JD, Symons JD. Activating P2Y1 receptors improves function in arteries with repressed autophagy. Cardiovasc Res. 2023 Mar 17;119(1):252-267. doi: 10.1093/cvr/cvac061. PMID: 35420120; PMCID: PMC10236004.

TULA-Family Regulators of Platelet Activation
Kunapuli SP, Tsygankov AY. TULA-Family Regulators of Platelet Activation. Int J Mol Sci. 2022 Nov 28;23(23):14910. doi: 10.3390/ijms232314910. PMID: 36499237; PMCID: PMC9736690.

Protein disulfide isomerase A1 as a novel redox sensor in VEGFR2 signaling and angiogenesis
Nagarkoti S, Kim YM, Ash D, Das A, Vitriol E, Read TA, Youn SW, Sudhahar V, McMenamin M, Hou Y, Boatwright H, Caldwell R, Essex DW, Cho J, Fukai T, Ushio-Fukai M. Protein disulfide isomerase A1 as a novel redox sensor in VEGFR2 signaling and angiogenesis. Angiogenesis. 2023 Feb;26(1):77-96. doi: 10.1007/s10456-022-09852-7. Epub 2022 Aug 19. PMID: 35984546; PMCID: PMC9918675.

Phosphorylation on Syk Y342 is important for both ITAM and hemITAM signaling in platelets Kostyak JC, Mauri B, Dangelmaier C, Vari HR, Patel A, Wright M, Reddy H, Tsygankov AY, Kunapuli SP. Phosphorylation on Syk Y342 is important for both ITAM and hemITAM signaling in platelets. J Biol Chem. 2022 Aug;298(8):102189. doi: 10.1016/j.jbc.2022.102189. Epub 2022 Jun 24. PMID: 35753354; PMCID: PMC9287148.

TULA-Family Regulators of Platelet Activation
Kunapuli SP, Tsygankov AY. TULA-Family Regulators of Platelet Activation. Int J Mol Sci. 2022 Nov 28;23(23):14910. doi: 10.3390/ijms232314910. PMID: 36499237; PMCID: PMC9736690.

Sex-related differences in the response of anti-platelet drug therapies targeting purinergic signaling pathways in sepsis
Amoafo EB, Entsie P, Albayati S, Dorsam GP, Kunapuli SP, Kilpatrick LE, Liverani E. Sex-related differences in the response of anti-platelet drug therapies targeting purinergic signaling pathways in sepsis. Front Immunol. 2022 Nov 2;13:1015577. doi: 10.3389/fimmu.2022.1015577. PMID: 36405709; PMCID: PMC9667743.

D121 Located within the DRY Motif of P2Y12 Is Essential for P2Y12-Mediated Platelet Function
Dangelmaier C, Mauri B, Patel A, Kunapuli SP, Kostyak JC. D121 Located within the DRY Motif of P2Y12 Is Essential for P2Y12-Mediated Platelet Function. Int J Mol Sci. 2022 Sep 29;23(19):11519. doi: 10.3390/ijms231911519. PMID: 36232816; PMCID: PMC9569438.

Eicosanoid-Activated PPARα Inhibits NFκB-Dependent Bacterial Clearance During Post-Influenza Superinfection
Lucarelli R, Gorrochotegui-Escalante N, Taddeo J, Buttaro B, Beld J, Tam V. Eicosanoid-Activated PPARα Inhibits NFκB-Dependent Bacterial Clearance During Post-Influenza Superinfection. Front Cell Infect Microbiol. 2022 Jul 4;12:881462. doi: 10.3389/fcimb.2022.881462. PMID: 35860381; PMCID: PMC9289478.

Phosphorylation on Syk Y342 is important for both ITAM and hemITAM signaling in platelets
Kostyak JC, Mauri B, Dangelmaier C, Vari HR, Patel A, Wright M, Reddy H, Tsygankov AY, Kunapuli SP. Phosphorylation on Syk Y342 is important for both ITAM and hemITAM signaling in platelets. J Biol Chem. 2022 Aug;298(8):102189. doi: 10.1016/j.jbc.2022.102189. Epub 2022 Jun 24. PMID: 35753354; PMCID: PMC9287148.

Evidence for a PI3-kinase independent pathway in the regulation of Rap1b activation downstream of the P2Y12 receptor in platelets
Dangelmaier C, Kunapuli SP. Evidence for a PI3-kinase independent pathway in the regulation of Rap1b activation downstream of the P2Y12 receptor in platelets. Platelets. 2022 Nov 17;33(8):1301-1306. doi: 10.1080/09537104.2022.2071855. Epub 2022 May 6. PMID: 35514261; PMCID: PMC9547944.

Clustering extent-dependent differential signaling by CLEC-2 receptors in platelets Dangelmaier C, Vari HR, Wright M, Kostyak JC, Kunapuli SP. Clustering extent-dependent differential signaling by CLEC-2 receptors in platelets. Res Pract Thromb Haemost. 2022 May 6;6(3):e12710. doi: 10.1002/rth2.12710. PMID: 35573643; PMCID: PMC9074038.

A novel role for endoplasmic reticulum protein 46 (ERp46) in platelet function and arterial thrombosis in mice
Zhou J, Wu Y, Rauova L, Koma G, Wang L, Poncz M, Li H, Liu T, Fong KP, Bennett JS, Kunapuli SP, Essex DW. A novel role for endoplasmic reticulum protein 46 (ERp46) in platelet function and arterial thrombosis in mice. Blood. 2022 Mar 31;139(13):2050-2065. doi: 10.1182/blood.2021012055. PMID: 34752599; PMCID: PMC8972093.

Aorta in Pathologies May Function as an Immune Organ by Upregulating Secretomes for Immune and Vascular Cell Activation, Differentiation and Trans-Differentiation-Early Secretomes may Serve as Drivers for Trained Immunity
Lu Y, Sun Y, Xu K, Saaoud F, Shao Y, Drummer C 4th, Wu S, Hu W, Yu J, Kunapuli SP, Bethea JR, Vazquez-Padron RI, Sun J, Jiang X, Wang H, Yang X. Aorta in Pathologies May Function as an Immune Organ by Upregulating Secretomes for Immune and Vascular Cell Activation, Differentiation and Trans-Differentiation-Early Secretomes may Serve as Drivers for Trained Immunity. Front Immunol. 2022 Mar 7;13:858256. doi: 10.3389/fimmu.2022.858256. PMID: 35320939; PMCID: PMC8934864.

29 m6A-RNA Methylation (Epitranscriptomic) Regulators Are Regulated in 41 Diseases including Atherosclerosis and Tumors Potentially via ROS Regulation - 102 Transcriptomic Dataset Analyses
Liu M, Xu K, Saaoud F, Shao Y, Zhang R, Lu Y, Sun Y, Drummer C 4th, Li L, Wu S, Kunapuli SP, Criner GJ, Sun J, Shan H, Jiang X, Wang H, Yang X. 29 m⁶A-RNA Methylation (Epitranscriptomic) Regulators Are Regulated in 41 Diseases including Atherosclerosis and Tumors Potentially via ROS Regulation - 102 Transcriptomic Dataset Analyses. J Immunol Res. 2022 Feb 15;2022:1433323. doi: 10.1155/2022/1433323. PMID: 35211628; PMCID: PMC8863469.

Synthesis, Biological Evaluation, and Computational Analysis of Biaryl Side-Chain Analogs of Solithromycin
Daher SS, Lee M, Jin X, Teijaro CN, Wheeler SE, Jacobson MA, Buttaro B, Andrade RB. Synthesis, Biological Evaluation, and Computational Analysis of Biaryl Side-Chain Analogs of Solithromycin. ChemMedChem. 2021 Nov 5;16(21):3368-3373. doi: 10.1002/cmdc.202100435. Epub 2021 Sep 3. PMID: 34355515; PMCID: PMC8571068.

A Bead Movement Based Computational Framework for 3-Dimensional Analysis of Biofilm Material Heterogeneity
Buttaro B, Queisser G. A Bead Movement Based Computational Framework for 3-Dimensional Analysis of Biofilm Material Heterogeneity. J Vis Exp. 2021 Jun 22;(172). doi: 10.3791/62454. PMID: 34251364.

Platelet Microparticles Enriched in miR-223 Reduce ICAM-1-Dependent Vascular Inflammation in Septic Conditions
Szilágyi B, Fejes Z, Rusznyák Á, Fenyvesi F, Pócsi M, Halmi S, Griger Z, Kunapuli SP, Kappelmayer J, Nagy B Jr. Platelet Microparticles Enriched in miR-223 Reduce ICAM-1-Dependent Vascular Inflammation in Septic Conditions. Front Physiol. 2021 May 31;12:658524. doi: 10.3389/fphys.2021.658524. PMID: 34135769; PMCID: PMC8201999.

Phosphorylation of protein kinase Cδ Tyr311 positively regulates thromboxane generation in platelets
Kostyak JC, Mauri B, Patel A, Dangelmaier C, Reddy H, Kunapuli SP. Phosphorylation of protein kinase Cδ Tyr311 positively regulates thromboxane generation in platelets. J Biol Chem. 2021 Jan-Jun;296:100720. doi: 10.1016/j.jbc.2021.100720. Epub 2021 Apr 28. PMID: 33932405; PMCID: PMC8164046.

Epidermal growth factor receptor association with β1-adrenergic receptor is mediated via its juxtamembrane domain
Patwa V, Guo S, Carter RL, Kraus L, Einspahr J, Teplitsky D, Sabri A, Tilley DG. Epidermal growth factor receptor association with β1-adrenergic receptor is mediated via its juxtamembrane domain. Cell Signal. 2021 Feb;78:109846. doi: 10.1016/j.cellsig.2020.109846. Epub 2020 Nov 22. PMID: 33238186; PMCID: PMC7775336.

Nitrate Is an Environmental Cue in the Gut for Salmonella enterica Serovar Typhimurium Biofilm Dispersal through Curli Repression and Flagellum Activation via Cyclic-di-GMP Signaling
Miller AL, Nicastro LK, Bessho S, Grando K, White AP, Zhang Y, Queisser G, Buttaro BA, Tükel Ç. Nitrate Is an Environmental Cue in the Gut for Salmonella enterica Serovar Typhimurium Biofilm Dispersal through Curli Repression and Flagellum Activation via Cyclic-di-GMP Signaling. mBio.2021 Feb 22;13(1):e0288621. doi: 10.1128/mbio.02886-21. Epub 2022 Feb 8. PMID: 35130730; PMCID: PMC8822344.

Interaction of the Joining Region in Junctophilin-2 With the L-Type Ca2+ Channel Is Pivotal for Cardiac Dyad Assembly and Intracellular Ca2+ Dynamics
Gross P, Johnson J, Romero CM, Eaton DM, Poulet C, Sanchez-Alonso J, Lucarelli C, Ross J, Gibb AA, Garbincius JF, Lambert J, Varol E, Yang Y, Wallner M, Feldsott EA, Kubo H, Berretta RM, Yu D, Rizzo V, Elrod J, Sabri A, Gorelik J, Chen X, Houser SR. Interaction of the Joining Region in Junctophilin-2 With the L-Type Ca²⁺ Channel Is Pivotal for Cardiac Dyad Assembly and Intracellular Ca²⁺ Dynamics. Circ Res. 2021 Jan 8;128(1):92-114. doi: 10.1161/CIRCRESAHA.119.315715. Epub 2020 Oct 23. PMID: 33092464; PMCID: PMC7790862.

P2Y12 antagonism results in altered interactions between platelets and regulatory T cells during sepsis
Albayati S, Vemulapalli H, Tsygankov AY, Liverani E. P2Y₁₂ antagonism results in altered interactions between platelets and regulatory T cells during sepsis. J Leukoc Biol. 2021 Jul;110(1):141-153. doi: 10.1002/JLB.3A0220-097R. Epub 2020 Nov 26. PMID: 33242353.53

Enhanced eradication of intracellular and biofilm-residing methicillin-resistant Staphylococcus aureus (MRSA) reservoirs with hybrid nanoparticles delivering rifampicin
Guo P, Xue HY, Buttaro BA, Tran NT, Wong HL. Enhanced eradication of intracellular and biofilm-residing methicillin-resistant Staphylococcus aureus (MRSA) reservoirs with hybrid nanoparticles delivering rifampicin. Int J Pharm. 2020 Nov 15;589:119784. doi: 10.1016/j.ijpharm.2020.119784. Epub 2020 Aug 30. PMID: 32877731; PMCID: PMC7854488

Loss of Protease-Activated Receptor 4 Prevents Inflammation Resolution and Predisposes the Heart to Cardiac Rupture After Myocardial Infarction Kolpakov MA, Guo X, Rafiq K, Vlasenko L, Hooshdaran B, Seqqat R, Wang T, Fan X, Tilley DG, Kostyak JC, Kunapuli SP, Houser SR, Sabri A. Loss of Protease-Activated Receptor 4 Prevents Inflammation Resolution and Predisposes the Heart to Cardiac Rupture After Myocardial Infarction. Circulation. 2020 Aug 25;142(8):758-775. doi: 10.1161/CIRCULATIONAHA.119.044340. Epub 2020 Jun 3. PMID: 32489148; PMCID: PMC9341277.

Protease-activated receptor 4 causes Akt phosphorylation independently of PI3 kinase pathways
Dangelmaier C, Kunapuli SP. Protease-activated receptor 4 causes Akt phosphorylation independently of PI3 kinase pathways. Platelets. 2021 Aug 18;32(6):832-837. doi: 10.1080/09537104.2020.1802415. Epub 2020 Aug 18. PMID: 32811251; PMCID: PMC7889752.

Iron alters the cell wall composition and intracellular lactate to affect Candida albicans susceptibility to antifungals and host immune response Tripathi A, Liverani E, Tsygankov AY, Puri S. Iron alters the cell wall composition and intracellular lactate to affect Candida albicans susceptibility to antifungals and host immune response. J Biol Chem. 2020 Jul 17;295(29):10032-10044. doi: 10.1074/jbc.RA120.013413. Epub 2020 Jun 5. PMID: 32503842; PMCID: PMC7380197.

ADP exerts P2Y12 -dependent and P2Y12 -independent effects on primary human T cell responses to stimulation
Vemulapalli H, Albayati S, Patwa VC, Tilley DG, Tsygankov AY, Liverani E. ADP exerts P2Y₁₂ -dependent and P2Y₁₂ -independent effects on primary human T cell responses to stimulation. J Cell Commun Signal. 2020 Mar;14(1):111-126. doi: 10.1007/s12079-019-00540-8. Epub 2019 Dec 5. PMID: 31808055; PMCID: PMC7176780.

M1-like macrophage-derived exosomes suppress angiogenesis and exacerbate cardiac dysfunction in a myocardial infarction microenvironment Liu S, Chen J, Shi J, Zhou W, Wang L, Fang W, Zhong Y, Chen X, Chen Y, Sabri A, Liu S. M1-like macrophage-derived exosomes suppress angiogenesis and exacerbate cardiac dysfunction in a myocardial infarction microenvironment. Basic Res Cardiol. 2020 Feb 28;115(2):22. doi: 10.1007/s00395-020-0781-7. PMID: 32112145.

Differential microRNA-21 and microRNA-221 Upregulation in the Biventricular Failing Heart Reveals Distinct Stress Responses of Right Versus Left Ventricular Fibroblasts
Powers JC, Sabri A, Al-Bataineh D, Chotalia D, Guo X, Tsipenyuk F, Berretta R, Kavitha P, Gopi H, Houser SR, Khan M, Tsai EJ, Recchia FA. Differential microRNA-21 and microRNA-221 Upregulation in the Biventricular Failing Heart Reveals Distinct Stress Responses of Right Versus Left Ventricular Fibroblasts. Circ Heart Fail. 2020 Jan;13(1):e006426. doi: 10.1161/CIRCHEARTFAILURE.119.006426. Epub 2020 Jan 9. PMID: 31916447; PMCID: PMC7006717.

TULA proteins as signaling regulators
Tsygankov AY. TULA proteins as signaling regulators. Cell Signal. 2020 Jan;65:109424. doi: 10.1016/j.cellsig.2019.109424. Epub 2019 Oct 19. PMID: 31639493.