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Lewis Katz School of Medicine

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Amit Rai, M.Sc., Ph.D.

Amit Rai, M.Sc., Ph.D.

Amit Rai, M.Sc., Ph.D.

  • Lewis Katz School of Medicine

    • Cardiovascular Sciences

      • Instructor

    • Aging + Cardiovascular Discovery Center

      • Instructor

Research Interests

Non-coding RNA's role in cardiovascular diseases

Education

  • Ph.D.,(Life Sciences): Jawaharlal Nehru University, New Delhi, India
  • M.Sc. (Biotechnology): VBS Purvanchal University, Jaunpur, UP, India

Memberships

  • American Heart Association 2021-present
  • The Academy for Cardiovascular Research Excellence-SAHR 2021-present

Awards and Honors

  • 2025-2028     AHA- Career Development Award 2024
  • First Place -Poster Competition- Post Doctoral division at the Mid-Atlantic Pharmacology Society (MAPS) of ASPET
  • 2024 Second Place- Oral Presentation Doctoral Symposium at Temple University,                  Philadelphia, PA.
  • 2024 Led team “RNA Renegades” as Project Leader to secure funding at national level by completing National Science Foundation-I Corps Hub (Northeast Region).
  • 2023 Outstanding presentation award at 20th Annual Spring research day at Emergency Medicine department of Ohio State University, Columbus, Ohio, USA.
  • 2022 First place in poster competition-2022 as student/trainee AHA-BCVS.
  • 2022 First place poster presentation at Asian Cardiovascular Society-SAHR. 2022 Second place award in poster category at 36th annual meeting of Ohio Physiological Society, which held at Ohio State University, Columbus, Ohio, USA.
  • 2022  American Heart Association-Post Doctoral Fellowship award.
  • 2015 First place in flow-cytometry quiz-competition in CSIR-CDRI- Beckman Coulter  Centre of Excellence in Flow Cytometry.
  • 2013 Qualified Indian Council of Medical Research- Junior Research Fellowship, Government of India, for admission in PhD program
  • 2012 Qualified Indian Council of Medical Research- Junior Research Fellowship, Government of India, for admission in PhD program
  • 2011-2012 Industrial trainee, Department of Biotechnology, Government of India, India.

Publications

PEER-REVIEWED ARTICLES (Original Research Article)

1. Kishore R, Gonzalez C, Cimini M, Mallaredy V, Benedict CL, Joladarashi D, Gurrala CT, Cheng Z, Trungcao M, Rai AK, Garikipati VN. Circular RNA Circ-Cdr1as modulates Macrophage phenotype and Cardiac Reparative Function by Circ-Cdr1as-miR-7-Klf4 pathway. bioRxiv. 2025:2025-02.

2. Stepanyan A, Brojakowska A, Zakharyan R, Hakobyan S, Davitavyan S, Sirunyan T, Khachatryan G, Khlgatian MK, Bisserier M, Zhang S, Sahoo S, Rai AK. Evaluating sex-specific responses to western diet across the lifespan: impact on cardiac function and transcriptomic signatures in C57BL/6J mice at 530 and 640/750 days of age. Cardiovascular Diabetology. 2024 Dec 28;23(1):454.

3. Rai AK, Muthukumaran NS, Nisini N, Lee T, Kyriazis ID, de Lucia C, Piedepalumbo M, Roy R, Uchida S, Drosatos K, Bisserier M. Transcriptome wide changes in long noncoding RNAs in diabetic ischemic heart disease. Cardiovascular diabetology. 2024 Oct 17;23(1):365.

4. Rai AK, Sanghvi S, Muthukumaran NS, Chandrasekera D, Kadam A, Kishore J, Kyriazis ID, Tomar D, Ponnalagu D, Shettigar V, Khan M. Role of mitochondrial ribosomal protein L7/L12 (MRPL12) in diabetic ischemic heart disease. Free Radical Biology and Medicine. 2024 Sep 1; 222:531-8.

5. Ahmad S, Pandey AR, Rai AK, Singh SP, Kumar P, Singh S, Gulzar F, Ahmad I, Sashidhara KV, Tamrakar AK. Moringa oleifera impedes protein glycation and exerts reno-protective effects in streptozotocin-induced diabetic rats. Journal of Ethnopharmacology. 2023 Apr 6; 305:116117.

6. Sahu N, Singh N, Arya KR, Reddy SS, Rai AK, Shukla V, Pandey J, Narender T, Tamrakar AK, Kumar B, Rajak BK. Assessment of the dual role of Lyonia ovalifolia (Wall.) Drude in inhibiting AGEs and enhancing GLUT4 translocation through LC-ESI-QTOF-MS/MS determination and in silico studies. Frontiers in Pharmacology. 2023 Mar 27; 14:1073327.

7. Rai AK, Rajan KS, Bisserier M, Brojakowska A, Sebastian A, Evans AC, Coleman MA, Mills PJ, Arakelyan A, Uchida S, Hadri L. Spaceflight-Associated Changes of snoRNAs in Peripheral Blood Mononuclear Cells and Plasma Exosomes—A Pilot Study. Frontiers in Cardiovascular Medicine. 2022:1645.

8. Goukassian D, Arakelyan A, Brojakowska A, Bisserier M, Hakobyan S, Hadri L, Rai AK, Evans A, Sebastian A, Truongcao M, Gonzalez C. Space flight associated changes in astronauts’ plasma‐ derived small extracellular vesicle microRNA: Biomarker identification. Clinical and Translational Medicine. 2022 Jun;12(6).

9. Sharma A, Singh S, Mishra A, Rai AK, Ahmad I, Ahmad S, Gulzar F, Schertzer JD, Shrivastava A, Tamrakar AK. Insulin resistance corresponds with a progressive increase in NOD1 in high fat diet- fed mice. Endocrine. 2022 May;76(2):282-93.

10. Bisserier M, Brojakowska A, Saffran N, Rai AK, Lee B, Coleman M, Sebastian A, Evans A, Mills PJ, Addya S, Arakelyan A. Astronauts plasma-derived exosomes induced aberrant EZH2-mediated H3K27me3 epigenetic regulation of the Vitamin D Receptor. Frontiers in cardiovascular medicine. 2022;9.

11. Bisserier M, Shanmughapriya S, Rai AK, Gonzalez C, Brojakowska A, Garikipati VN, Madesh M, Mills PJ, Walsh K, Arakelyan A, Kishore R. Cell‐Free mitochondrial DNA as a potential biomarker for astronauts' health. Journal of the American Heart Association. 2021 Nov 2;10(21): e022055.

12. Gupta S, Rai AK, Pandey S, Singh LR, Kant R, Tamrakar AK, Sashidhara KV. Microwave-assisted efficient synthesis of pyrazole-fibrate derivatives as stimulators of glucose uptake in skeletal muscle cells. Bioorganic & Medicinal Chemistry Letters. 2021 Feb 15; 34:127760.

13. Rai AK, Jaiswal N, Maurya CK, Sharma A, Ahmad I, Ahmad S, Tamrakar AK. Fructose-induced AGEs-RAGE signaling in skeletal muscle contributes to impairment of glucose homeostasis. The Journal of Nutritional Biochemistry, (2019) 71, 35-44.

14. Rai AK, Singh SP, Pandey AR, Ansari A, Ahmad S, Sashidhara KV, Tamrakar AK. Flavonoids from Polyalthia longifolia prevents advanced glycation end products formation and protein oxidation aligned with fructose-induced protein glycation. Natural Product Research. 2019 Oct 3:1-5.

15. Sharma A, Maurya CK, Arha D, Rai AK, Singh S, Varshney S, Schertzer JD, Tamrakar AK. Nod1- mediated lipolysis promotes diacylglycerol accumulation and successive inflammation via PKCδ- IRAK axis in adipocytes. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease. 2019 Jan 1;1865(1):136

16. Arha D, Ramakrishna E., Gupta AP, Rai AK, Sharma A, Ahmad I, Riyazuddin M, Gayen, JR, Maurya R. and Tamrakar AK. Isoalantolactone derivative promotes glucose utilization in skeletal muscle cells and increases energy expenditure in db/db mice via activating AMPK-dependent signaling. Molecular and cellular endocrinology, 460 (2018): 134-151.

17. Jaiswal N, Maurya CK, Pandey J, Rai AK & Tamrakar AK. Fructose-induced ROS generation impairs glucose utilization in L6 skeletal muscle cells, Free Radical Research, (2015) 49:9, 1055- 1068.

18. Maurya CK, Arha D, Rai AK, Kant Kumar S, Pandey J, Avisetti DR, Kalivendi SV, Klip A, Tamrakar AK. NOD2 activation induces oxidative stress contributing to mitochondrial dysfunction and insulin resistance in skeletal muscle cells. Free Radic. Biol. Med. (2015) 89, 158–169.

19. Khan MF, Maurya CK, Dev K, Arha D, Rai AK, Tamrakar AK, & Maurya R. Design and synthesis of lupeol analogues and their glucose uptake stimulatory effect in L6 skeletal muscle cells. Bioorganic & medicinal chemistry letters, (2014), 24(12), 2674-267.

OTHER ARTICLES (Review)

1. Jagielski NP, Rai AK, Rajan KS, Mangal V, Garikipati VN. A Contemporary Review of snoRNAs in Cardiovascular Health: RNA Modification and Beyond. Molecular Therapy-Nucleic Acids. 2023 Dec 5.

2. Rai AK, Lee B, Hebbard C, Uchida S, Garikipati VN. Decoding the complexity of circular RNAs in cardiovascular disease. Pharmacological Research. 2021 Sep 1; 171:105766.

3. Rai AK, Lee B, Gomez R, Rajendran D, Khan M, Garikipati VN. Current Status and Potential Therapeutic Strategies for Using Non-coding RNA to Treat Diabetic Cardiomyopathy. Frontiers in Physiology. 2021 Jan 22; 11:1750.

4. Tamrakar AK, Maurya CK, Rai AK. PTP1B inhibitors for type 2 diabetes treatment: a patent review (2011-2014). Expert Opinion on Therapeutic Patents (2014), 24 (10): 1101.

BOOKS, MONOGRAPHS, CHAPTERS

1. Rai AK and Garikipati VN. Quantitative Real-Time PCR for Circular RNA Detection and Analysis. Cardiomyocytes (Methods and Protocols), Jan 2025

2. Rai AK and Garikipati VN. Detection of small nucleolar RNAs by quantitative real time PCR. Cardiomyocytes (Methods and Protocols), Jan 2025.

3. Lee B, Kyriazis ID, Patil R, Alvi SB, Rai AK, Khan M, Garikipati VN. Immune Responses Regulated by Exosomal Mechanisms in Cardiovascular Disease. In Immune Cells, Inflammation, and Cardiovascular Diseases, 2022 (pp. 187-214). CRC Press.

ABSTRACTS  (Note whether presented platform, presented poster or published and not presented)

1. Rai A, Rajan KS, Bisserier M, Brojakowska A, Sebastian A, Evans A, Coleman M, Mills P, Arakelyan AA, Uchida S, Hadri L. Abstract P1115: SnoRNAs As Potential Biomarkers For Assessment Of Health Risks In Astronauts. Circulation Research. 2022 Aug 5;131(Suppl_1):AP1115-. (American Heart Association: BCVS- July 2022): Poster Presentation.