Karim Bahmed, PhD
Instructor (Research), Department of Thoracic Medicine and Surgery
- About Me
- Idiopathic Pulmonary Fibrosis (IPF) and Emphysema
Cellular DNA is most frequently targeted by oxidative stress which may lead to DNA lesions. Continuous oxidative DNA damage can contribute to the pathophysiology of various lung diseases such as Emphysema and Idiopathic Pulmonary Fibrosis (IPF). Emphysema is one of the diseases that comprise the term COPD (chronic obstructive pulmonary disease) and consists of a unique pattern of alveolar wall destruction. IPF is characterized by a progressive decline in lung function due to scarring of lung tissue. Emphysema and IPF are not curable. We isolate primary alveolar type II (ATII) cells from control lungs and excess tissue obtained from lung transplants of patients with emphysema and IPF to study their injury. Control ATII cells use different mechanisms to repair DNA damage to preserve their genomic integrity. However, if not repaired, DNA damage may lead to double strand breaks, chromosomal rearrangement, and genetic instability, which causes cell death and lung tissue damage. We are particularly interested in understanding the mechanisms of higher DNA damage and lower DNA repair in ATII cells in emphysema and IPF in comparison with controls. Our research focuses on the role of selected genes and proteins involved in these processes and identified by RNA sequencing and mass spectrometry analysis performed in these ATII cells. We are using in vitro, ex vivo and in vivo approached to elucidate the function of these molecules in the maintenance of the genomic DNA and cellular survival. Our goal is to understand their role in ATII cell injury in emphysema and IPF, which may lead to novel potential therapeutic approaches.
- Education, Training & Credentials
- Postdoctoral Fellowship, Department of Biochemistry and Molecular Genetics, University of Colorado, Denver, CO, 2013
- Postdoctoral Fellowship, Department of Molecular Pharmacology, St. Jude Research Hospital, Memphis TN, 2010
- PhD, Biology, University of Nancy 1, Nancy, France, 2003
- MS, Microbiology-Enzymology and Nutrition, University of Nancy 1, Nancy, France, 1998
- BS, Biochemistry, University of Nancy 1, Nancy, France, 1997
- DNA Repair Group
- Anna Rogojina, Stefan Gajewski, Karim Bahmed, Neil Osheroff, John L. Nitiss, Topoisomerase II Inhibitors: Chemical Biology, In: DNA Topoisomerases and Cancer. Cancer Drug Discovery and Development (Ed. Yves Pommier), 2012, Springer Verlag, pp. 211-243.
- Karim Bahmed, Roger Bonaly, Said Benallaoua and Joel Coulon, 2005, Effect of sub- inhibitory concentrations of amphotericin B on the yeast surface and phagocytic killing activity, Process Biochemistry 40 (2): 759-765.
- Karim Bahmed, Fabienne Quiles, Roger Bonaly, Michel Wathier, Bernard Pucci and Joel Coulon, 2005, Dansyl N-Acetyl Glucosamine as precursor of fluorescent Chitin: Method to analyse chitin Synthase. Process Biochemistry 40 (7): 2523-2529.
- Nadia Maazouzi, Karim Bahmed, Salem Chakir, Fabienne Quiles, Roger Bonaly and Joel Coulon, 2005, Chemical modification and stability of the cell wall phosphopeptidomannans of flocculent and weakly flocculent Kluyveromyces bulgaricus cells, Process Biochemistry 40 (1): 313-319.