In This Section

Instrumentation

Analyzers

Calibur

  • 2 lasers (488 nm and 633 nm), 4 fluorescent channels
  • Analog acquisition using CellQuest Pro software, online compensation only
  • Detector configuration

LSR II

  • 4 lasers (488 nm, 407 nm, 633 nm, 355 nm), 13 fluorescent channels
  • Digital acquisition using DiVa software, automatic offline compensation
  • Uses fiber optics and Octagon/Trigon detector arrays to increase sensitivity
  • Detector configuration

Sorters

Aria IIµ

  • 3 lasers (488 nm, 407 nm, 633 nm), 8 fluorescent channels
  • Digital acquisition using DiVa software, automatic offline compensation
  • Uses fiber optics and Octagon/Trigon detector arrays to increase sensitivity
  • Cuvette-based flow cell, more sensitive than jet-in-air cytometer
  • Up to 4-way sorting, single cell sorting into 96 well plates
  • Nozzle size and sorting pressure can be changed for different types of cells. Available nozzles: 70 µm, 85 µm, 100 µm.  Larger nozzle size results in lower sheath pressure, and the cells are less stressed. However, the sort is slower and collected cells are in greater volume of PBS.
  • Detector configuration

Influx with HEPA-filtered enclosure

  • 4 lasers (488 nm, 407 nm, 633 nm, 561 nm), 14 fluorescent channels
  • Analog to digital acquisition
  • 561 nm laser excites PE and PE tandem dyes better than 488 nm laser.  It also excites red fluorescent proteins, such as mCherry, DsRed.
  • Forward Scatter detector is Photo Multiplier Tube (PMT) instead of Diode, more sensitive for particles < 1 µm
  • Jet-in-air cytometer
  • Up to 4-way sorting, single cell sorting into 96 well plates
  • Nozzles: 70 µm, 100 µm, 140 µm, 200 µm.
  • BSL-2 sorting including live human cells
  • Detector configuration

Analysis Workstation

  • Mac Pro computer with Flowjo and CellQuest Pro software
  • CD/DVD writer